Introduction to Invitrogen 1 kb Plus Ladder: What it is and What it Does
The Invitrogen 1 kb Plus DNA Ladder is a purified mixture of linear double-stranded DNA fragments that range from 500 bp to 9500 bp in increments of 100 bp. This ladder is an incredibly useful tool for molecular biologists and biochemists who need it to accurately measure and compare the sizes of Double-Stranded or Single-Stranded DNA fragments after they have been subjected to Molecular Cloning or PCR. The Invitrogen 1 kb Plus Ladder allows users to approximate the size of unknown DNA fragments within a sample by comparing them to markers comprised of known lengths present in the ladder. Depending on the method and scale used, measurements can be accurate within bands as small as 10 base pairs apart.
This kit contains 6 tubes with pre-diluted Purified ReadyLadders which have a concentration equal to 50 ng/µl and no further dilution necessary. Simply load your samples on top of readymade agarose gels alongside one tube of each readymade ladder, run your experiment, photograph the result with a UV transilluminator, and you’re good to go! One major benefit this kit provides is convenience as its ready-to-use mixtures are already proven to be accurate before they reach you here. Furthermore, 5 additional tubes contain 4 different colored dyes which provide reference points on any pictures taken so that sizing accuracy is optimised and results can be quickly compared between different runs with ease.
Taken together, these features all contribute towards making the Invitrogen 1 kb Plus DNA Ladder an indispensible tool for any laboratory interested in working extensively with Variably Sized Nucleic Acids present in multiple experimental sets from different species, especially during Early Stages in Molecular Cloning Protocols where varying fragment sizes are expected but need measuring first for accuracy’s sake before proceeding onwards.
Benefits of Using Invitrogen 1 kb Plus Ladder for Accurate Molecular Weight Estimation
Invitrogen 1 kb Plus Ladder is an ideal solution for accurate molecular weight estimation because of its numerous advantages. On top of the obvious convenience of having a ready-made ladder with markers ranging from 1 to 11 kb, the 1kb Plus Ladder also allows researchers to easily back calculate or ‘ladder up’ the size of their sample. This makes it easier and quicker to find out the molecular weight compared to creating one’s own ladders from separate individual molecular mass markers.
The Invitrogen 1 kb Plus Ladder also has other benefits that can make life easier for researchers in the lab. The pre-mixed solution allows for an easy and quick workflow; no time-consuming mixing necessary! Additionally, each Ladder contains pre-quantified amounts which enables fast set up and results in minimal waste. Unlike many other DNA sizing ladders on the market, Invitrogen’s 1kb Plus offers high intensity bands as well as indelible fluorescent labels that enable effortless visualization without so much as touching a pipette.
Lastly, another advantage of using Invitrogen’s 1kb Plus is its accuracy; when used properly, it provides reliable estimates down to 0.1 kb resolution with even a single band on larger sizes like 9kb and above! All in all, taking into consideration everything from speediness to precision, and affordability too – whatever your research may be working with – there’s no denying that Invitrogen’s 1 kb plus ladder is one great option for any researcher looking to accurately estimate their sample’s molecular weights quickly and economically!
Step-by-Step Instructions for Using Invitrogen 1 kb Plus Ladder
Welcome to the guide to using Invitrogen 1 kb Plus Ladder! This step-by-step tutorial will walk you through how to properly use this essential tool for successful size confirmation of DNA fragments.
Before getting started, let’s quickly go over what exactly an Invitrogen 1kb Plus Ladder is and why it is so important. The ladder consists of eleven bands of an orderly arrangement of DNA fragments in sizes ranging from 2000bp to 250bp. It provides a reference marker for accurately measuring the mass and length of unknown samples, which in turn allows scientists make assessments about their genomic sequences.
So now that we know what it’s all about, let’s get started:
Step 1: Get your materials together. Make sure you have everything before starting; this will save you time and hassle later on. You will need 15ul 8X loading dye, 5ul DNA sample, a 100 bp ladder (Invitrogen 1kb Plus Ladder or similar), loading buffer, both PCR tubes and reaction tubes containing the same sample, electrophoresis equipment (a power supply unit and appropriate agarose gel), spectrophotometer and sterile pipettes. Depending on your research needs additional equipment might be needed; don’t forget to bring it too!
Step 2: Run PCR reactions using your normal protocols on both sets of PCR tubes and store them at 4°C until ready to use. Both should contain identical samples but with different concentrations; this will ensure accurate comparison between samples during size confirmation processes.
Step 3: Measure absorbance values with a spectrophotometer to determine if any concentrations have been lost during storage or preparation processes. This step helps avoid possible contamination problems due to improper handling; if concentrations are found too low repeat Step 2 until acceptable amounts are reached then proceed onto next step.
Step 4: Load equal volumes into each lane
Common Questions & Answers about Invitrogen 1 kb Plus Ladder
What is the Invitrogen 1 kb Plus DNA ladder?
The Invitrogen 1 kb Plus DNA ladder is a ready-to-use collection of 11 double-stranded DNA fragments that are useful for tracking migration during agarose gel electrophoresis. The premixed ladder contains 24 different bands with sizes evenly distributed between 1-10 kb in 100 bp intervals. The marks are stable and produce a uniformly colored pattern on ethidium bromide stained agarose gels, providing an accurate size standard for routine analysis of nucleic acids from PCR or restriction digests.
What does the Invitrogen 1 kb Plus ladder contain?
The Invitrogen 1 kb Plus ladder contains 11 double stranded DNA fragments ranging in size from 1 to 10kB that when used as standards help in accurately identifying the size of samples being run on an agarose gel electrophoresis. In each 50 μl reaction, five popular band sizes are provided: 2, 3, 4, 6, and 8 kW giving researchers an economical alternative to gel purification profiling.
Is the Invitrogen 1kb Plus Ladder pre single stranded or double stranded?
The Invitrogen 1kb Plus Ladder is double stranded and can be resolved as such following electrophoresis on an agarose gel. The features of this formulation include uniformity between bands resulting in comparable intensities allowing for easier visual analyzing upon viewing gels.
How will using the Invitrogen1kb plus Ladder benefit me?
Using the methylene blue dye provides remarkable convenience and performance benefits over traditional manual loading methods during preparation of your samples prior to running them on a gel. It eliminates guesswork associated with manually preparing cues like density marker solution or boiling molecular weight marker solutions which makes any processes you conduct within your lab more efficient and reliable than ever before! Additionally, it has been optimized so that you get uniform interaction between bands enabling easier visualization upon
Five Interesting Facts about Invitrogen 1 kb Plus Ladder
The Invitrogen 1 kb Plus Ladder is an essential laboratory tool for accurate, consistent and reliable DNA analysis. Here are five interesting facts about this product:
1. The Invitrogen 1 kb Plus Ladder includes ready-to-use DNA fragments of different sizes that have been accurately sized and precisely separated by analytical sizing columns, which allows hassle-free preparation of high quality samples for applications such as PCR, DNA sequencing, electrophoresis and hybridization experiments.
2. This ladder consists of 12 distinct double-stranded DNA bands ranging from 500 bp to 10,600 bp separation between each band with relatively even size increments that facilitate the rapid identification of target nucleic acid amplicons during molecular cloning or sequence mapping studies.
3. Unlike other DNA ladders on the market, the Invitrogen 1kb Plus Ladder also contains a positive control that indicates how well samples are being loaded or run in agarose gels due to its fluorescent dye incorporated into its manufacturing process when it comes in contact with light energy.
4. The addition of the RNAse inhibitor helps to reduce any secondary degradation associated with enzymatic activity commonly associated with long-term storage methods meaning users can store the ladder for up to one year at -20°C without a noticeable decrease in molecular weight or any visual changes over time compared with freshly prepared ladders from Invitrogen.
5. Finally this ladder was created using Sigma Life Science’s Precision Size Standards ensuring uniformity within each tube allowing users consistent fragment spacing over time making it perfect for cutting down on sample preparation troubleshooting processes as well as enhancing reproducibility amongst experiments across labs worldwide!
Conclusion: Summarising the Benefits of Using Invitrogen 1 kb Plus Ladder
Invitrogen 1 kb Plus Ladder is a powerful tool for molecular biology applications, offering great value for the cost. It allows researchers to accurately measure, detect and compare DNA fragments with known sizes in the range of 0.1 kb to 10 kb. Its ready-to-use premixed solution includes six blue and three orange dyes, eliminating the need for tedious gel staining procedures. The user can quickly determine the concentration and size of their DNA samples based on the fluorescent intensity bands on the ladder, providing a faster workflow and saving time in experiments. It also contains low amounts of contaminants such as RNA and protein so that it can be used even with sensitive downstream applications such as PCR, sequencing or blotting. On top of all this, Invitrogen 1 kb Plus Ladder is an economical way to get maximal performance since higher concentrations are supplied at lower prices per unit than competing ladders designed for similar applications. For these reasons, Invitrogen 1kb Plus Ladder is an indispensable item in any research laboratory striving toward greater accuracy and efficiency in its biological experiments.