Introduction to Pageruler Prestained Protein Ladders
Organizing and tracking proteins in research can often be a complicated and time-consuming process. To make this job a bit easier, analytical chemists have developed the Pageruler Prestained Protein Ladder – an incredibly useful tool that conveniently measures proteins on polyacrylamide gels. By using this protein ladder, researchers can quickly identify and measure the size of proteins through visual band identification on the gel.
The Pageruler Prestained Protein Ladder is composed of 10 different numbered bands with each band representing a well-defined size range. The ladder is prestained so that when it’s run along with other unknown proteins during electrophoresis, scientists can clearly see the markers by their color or fluorescence intensity. This makes for easy visualization of various protein sizes along with their corresponding molecular weights.
Each Pageruler Prestained Protein Ladder features 11 bands ranging from Mw 10 to 187 kD that are precisely calibrated to give you accurate molecular weight determinations throughout your experiments. Each marker satisfies all laboratory safety requirements, contains nonionic detergents and uses dyes which are not hazardous or mutagenic per ASTM D4236 standards (Directive 2000/45/EC). Plus, these ladders provide consistent results operation after operation!
The Pageruler Prestained Protein Ladders provide a simple way to quickly determine protein size and also serve as an internal control for normalizing migration speeds in electroblotting applications too! So whether you’re researching cancer biology, immunology or metabolic pathways, the Pageruler line of products is sure to meet all your molecular biology needs in one convenient package!
Benefits of Using Pageruler Prestained Protein Ladder for Accurate Sizing
Pageruler Prestained Protein Ladder is a reliable, accurate tool for sizing proteins in various forms of analysis, making it an essential part of any laboratory’s equipment. This ladder clearly marks sizes on each step in the range from 10 to 245 kilodaltons (kDa), allowing for easy identification and comparison of molecular weights. Pageruler features a unique visual marker system that combines blue, orange and green colors at varying sizes to provide better accuracy than standard painted dyes. This system also increases precision when analyzing complex mixtures or low concentrations of samples, meaning users can rest assured that they are getting clear, precise results every time. Additionally, this ladder co-migrates well with other fluorescently labeled protein ladders or markers—enabling researchers to size multiple proteins simultaneously in the same gel runs.
Using Pageruler not only saves you time due to its improved accuracy and high resolution visuals but it also comes pre-stained rather than needing manual staining after running electrophoresis– eliminating potential user error while saving even more time. Each pack contains 8 individual ladders with 5 µl each providing ample quantity for a range of experiments; there is no need to buy additional reagents needed for conventional staining methods as the dye is incorporated into the formulation which simplifies both your workflow and budgeting.
Finally, Pageruler Prestained Protein Ladder cuts down on waste as any unused portion can be stored long term in -20℃ recommended storage temperature–allowing you several options depending on the frequency you run your assay (you won’t have to buy new each time!). Overall you get convenience, accuracy and reduction in costs due to product design without compromising data quality —resulting in sound scientific decisions based on dependable data when using PageRuler Prestained Protein Ladder!
Step-by-Step Guide for Proper Usage of the Pageruler Prestained Protein Ladder
1. Begin by selecting the correct ladder concentration for your experimental needs – The Pageruler Prestained Protein Ladder is offered in a range of concentrations, from 0.5 to 6 mg/mL, to fit different molecular weight ranges and experiment types. To select the appropriate ladder for your experiment, you need to review the molecular weight markers and total number of bands provided for each ladder type.
2. Prepare samples for loading – To prepare each sample for loading onto the gel, thoroughly mix sample composition with a compatible running buffer or sample buffer solution (e.g., Tris–Glycine or SDS). For best results, use 2-4 μL for the highest band intensity during electrophoresis.
3. Load and run electrophoresis – First, load 5-6 μL of proper Pageruler Prestained Protein Ladder into one well of an agarose gel already laid out in a casting stand with an included marker dye solution as a reference point and run at 100 V until bromophenol blue has travelled all the way through the gel (typically 1-2 hours). Then add appropriate samples that have been mixed in either running buffer or sample buffer solution on separate wells in neat rows across the gel with each row no more than 8 wells wide; again run at 100 V until bromophenol blue has traveled all the way through the gel (typically 1-2 hours). When using preparatory gels prior to transfer, it is recommended to set up a reference lane along with any other samples being loaded onto the preparatory gels so as to compare post transfer/staining/imaging results with their initial loading positions on the preparatory gels. Note: The Pageruler Prestained Protein Ladder can also be used directly on Western blots without prerunning electrophoresis gels prior to transfer if desired.
4. Visualize proteins on gel – Once
Commonly Asked Questions about Pageruler Prestained Protein Ladders
What Is the Pageruler Prestained Protein Ladder?
The Pageruler Prestained Protein Ladder is a ready-to-use gel electrophoresis system for analyzing and visualizing protein samples. It’s pre-stained, making it easy to monitor progress during electrophoretic separation in real time. This ladder consists of ten well defined, calibrated protein bands that run at specific molecular weights (in Kda) between 10 and 247 Kda. Samples are separated according to both electrical charge and size, allowing scientists to estimate the molecular weight of their proteins and other biomolecules. The ladder can be used with all types of resolving gels (SDS-PAGE or Tricine gels). Each lot is QC tested for accurate sizing and integrity.
What Are Some Benefits of Using the Pageruler Prestained Protein Ladder?
Using the Pageruler Prestained Protein Ladder offers several advantages over traditional formaldehyde staining methods; the most notable being time savings since there is no need to prepare your own protein stain solution or wait overnight for results. Additionally, it includes preprepared markers that make it ideal for plasma sample analysis as they contain a broad spectrum of sizes which effectively cover typical plasma proteins like albumin, fibrinogen, immunoglobulin etc. Furthermore, as this system permits simultaneous sample loading and running, users will also be able to save on loading buffer volumes compared to traditional marking formats. Moreover, its accurately sized components provide reliable calibration standards for fractionation control during 2D gel electrophoresis or proteomic analysis applications. Finally, due to its non toxicity status even hazardous material handlers do not need any extra safety protocols when using this product thereby saving considerable amount of instrumentation costs because you don’t have acquire any additional equipments like gloves or protective glasses during experimentation.
How Does One Use the Pageruler Prestained Protein Ladder?
Top 5 Facts About Pageruler Prestained Protein Ladders
Pageruler Prestained Protein Ladders are an important tool in biologists and biochemists’ labs. Here are 5 facts to know about these ladders:
1. Pageruler Prestained Protein Ladders come in a wide range of sizes, from 5 to 247 kDa. This makes them an ideal choice for analyzing proteins of different molecular weights, giving researchers more flexibility when examining samples.
2. Because the ladders are pre-staines, they can be analyzed immediately with gel electrophoresis using any UV transilluminator or imaging system that is sensitive to ultraviolet light. This saves precious time and money by eliminating the need to stain samples before running a gel.
3. The proteins in the ladders are labeled with a blue dye specifically designed for optimal intensities for all levels of exposure, allowing for precision visualization under a variety of conditions—bright or low lighting environments, varying intensity levels or even short exposures up to one minute long.
4. Unlike unstained protein ladders that rely on secondary stains after loading onto an agarose gel, the Pageruler Prestained Protein Laddert has already been stained so it is ready right out of the tube – no need for further staining procedures!
5. Pageruler Prestained Protein Ladders comes with features like BCA calibration markers help ensure precise mass measurements and consistent results between assays and laboratories – yet another way it helps make protein analysis easier than ever before!
Conclusion – Final Considerations Regarding Accurately Measuring Proteins with the Pageruler Ladder
The Pageruler Ladder is a useful tool for accurately measuring proteins in certain applications. With its simple design and its ability to easily detect small stains, it offers an easy and cost-effective solution to accurately measure protein samples. Furthermore, the ladder’s built-in markings help ensure that measurement results are consistent and reliable. As such, the Pageruler Ladder can be a helpful resource for laboratory technicians or researchers who need to measure proteins quickly and efficiently.
Despite its accuracy and dependability, there are a few things to consider when using the Pageruler Ladder to measure proteins. First of all, users should make sure that the sample size is appropriate for comparison with the ladder; too large or too small of samples can skew results significantly. Additionally, users should always confirm their measurements by running control samples; this will help eliminate any chance of error due to factors like electrophoresis conditions or gel manufacturing defect. Finally, users should also remember that Protein concentration data collected with the Pageruler Ladder can vary from other methods as each one provides unique quantification ranges and has their own respective strengths and weaknesses; thus it is important for users to select which method best fit their needs before starting an experiment.
In conclusion, the Pageruler Ladder is an excellent resource for measuring proteins in certain applications; however, careful consideration must be taken into account when determining whether it is appropriate for any given situation as well as ensuring an accurate readout before relying on these results.